Mississippi Valley Airlines was a regional air carrier serving the Upper Midwestern region of the United States. Originally founded as Gateway Aviation, the carrier initially had its headquarters in La Crosse, Wisconsin and began scheduled flight operations on July 22, 1968. The carrier name changed to Mississippi Valley Airways in October 1969, later becoming Mississippi Valley Airlines, with the headquarters moving to Moline, Illinois in January 1982. The airline merged with Air Wisconsin 1985 and thus lost its previous identity.